ADP 유도 P2Y1단백질에 의한 사람치아 치수세포의 분화 조절
ADP-Induced P2Y1 Regulates the Differentiation of Human Dental Pulp Cells
문서윤, 최장열, 안상건,
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문서윤 ( Moon Seo-Yun ) - 조선대학교 치과대학 구강병리학교실
최장열 ( Choi Jang-Yeol ) - 조선대학교 치과대학 구강병리학교실
안상건 ( Ahn Sang-Gun ) - 조선대학교 치과대학 구강병리학교실
KMID : 0829220170410030105
Abstract
To gain insights into the role of purinergic receptors in human dental pulp cells (hDPCs) differentiation, we characterized the expression and functional activity of P2Y1 receptors and investigated the effects of ADP on the proliferation and differentiation of this pulp stem-like cell population. Our data showed that ADP did not induce cell proliferation to expose the various ADP concentrations for 72 hours, but the proliferative capacity of hDPCs was inhibited at higher ATP concentrations (100 μM). Using RT-PCR analysis, we found that ADP induced several P2Y receptors including P2Y1 as well as odontoblastic differentiation genes, dentin matrix protein 1 (DMP1) and dentin sialophosphoprotein (DSPP) in a dose-dependent manner. The effects of ADP on the expression of DMP-1 and DSPP mRNA were prevented by the P2Y1 antagonist MRS2179. The extracellular matrix calcium deposits were clearly observed in ADP-treated hDPCs by alizarin red S staining. Quantitative measurement of mineralization induced by ADP was significantly inhibited in MRS2179-treated hDPCs. These results may provide new insights into the molecular regulation of the differentiation of hDPCs.
키워드
ADP ; P2Y1 ; Differentiation ; Human dental pulp cells
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